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NIES collection MICROBIAL CULTURE COLLECTION General information Search on strains data List of strains Taxonomic index Alphabetical index How to order Cart Japanese HOME Search Strain Data Strain Data Strain Data Strain Data Reference --> Strain number NIES-324   Phylum Heterokontophyta   Class Bacillariophyceae   Scientific name Skeletonema marinoi-dohrnii complex   Synonym   Former name Skeletonema costatum (Greville) Cleve   Common name Centric diatom   Locality (Date of collection) Osaka Bay Off Kobe, Hyogo, Japan (1985-07-02)   Latitude / Longitude   Habitat (Isolation source) Marine (Seawater)   History Isolator (Date of isolation) Yamochi, Susumu (1985-07-10)   Identified by Yamochi, Susumu   State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]   Culture condition (Preculture condition) Medium:  f/2   Temperature:  15 C Light intensity:  8 µmol photons/m2/sec, L/D cycle:  10L:14D Duration:  1 M   Gene information 18S rRNA ( AB488611 )   Cell size (min - max) 3 - 10 μm   Organization Unicellular  Characteristics Red tide ; Test strain (Khanam et al. 2017) ; Planktonic ; DNA sequencing indicates this strain is included in Skeletonema marinoi/dohrnii clade   Other strain no. Other strain no. : Sk-85su   Remarks Axenic  Movie   Reference Yamaguchi, H., Sumida, N., Matsukawa, K., Adachi, M., Tomaru, Y., Ishikawa, K., Hotta, J. 2023 Establishment of a simple method for cryopreservation of the marine diatoms, Chaetoceros and Phaeodactylum. J. Appl. Phycol., 33, 2285–2293. Keywords: CELLBANKER; Cryopreservation; Marine diatoms; Chaetoceros tenuissimus; Phaeodactylum tricornutum; Transgenic diatom Strain(s): 324,  3714,  3715  DOI: 10.1007/s10811-023-03040-w Yamasaki, Y., Kameo, S., Wada, Y., Kitatsuji, S., Yuasa, K., Shikata, T. 2023 Toxicological risk assessment of harmful algal species using the rotifer exposure test. Bull. Plankton Soc. Japan, 70, 52-61 (in Japanese with English summary). Keywords: Brachionus plicatilis; Dinophyceae; harmful algal bloom species; lethal effect on rotifers; Raphidophyceae Strain(s): 1,  324  DOI: 10.24763/bpsj.70.2_52 Onduka, T., Kono, K., Ito, M., Ohkubo, N., Hano, T., Ito, K., Mochida, K. 2020 Ecological risk assessment of an antifouling biocide triphenyl (octadecylamine) boron in the Seto Inland Sea, Japan. Mar. Pollut. Bull., 157, 111320 (article ID). Keywords: Algal growth inhibition test; Crustacean reproduction test; Early-life stage toxicity test; Species sensitivity distribution Strain(s): 324  PubMed: 32658685 DOI: 10.1016/j.marpolbul.2020.111320 Hikihara, R., Yamasaki, Y., Shikata, T., Nakayama, N., Sakamoto, S., Kato, S., Hatate, H., Tanaka, R. 2020 Analysis of phytosterol, fatty acid, and carotenoid composition of 19 microalgae and 6 bivalve species. J. Aquat. Food Prod. Technol., 29, 461-479. Keywords: Microalgae; bivalve; phytosterol; fucosterol; fatty acid Strain(s): 1,  10,  324  DOI: 10.1080/10498850.2020.1749744 Hamamoto, Y. & Honda, D. 2019 Nutritional intake of Aplanochytrium (Labyrinthulea, Stramenopiles) from living diatoms revealed by culture experiments suggesting the new prey–predator interactions in the grazing food web of the marine ecosystem. PLoS One, 14, e0208941 (article ID). Strain(s): 324,  3712  PubMed: 30625142 DOI: 10.1371/journal.pone.0208941 Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8,  254,  274,  286,  324,  331,  353,  381,  494,  623,  624,  997,  1012,  1013,  1015,  1320,  1330,  1333,  1334,  1335,  1372,  1377,  1388,  1390,  1396,  1397,  1408,  1438,  1439,  1441,  1868,  1964,  2008,  2142,  2144,  2149,  2300,  2305,  2325,  2433,  2498,  2502,  2566,  2584,  2586,  2589,  2590,  2677,  2878,  3356,  3374,  3775,  3876  PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0 Khanam, MR., Shimasaki, Y., Hosain, MZ., Mukai, K., Tsuyama, M., Qiu, X., Tasmin, R., Goto, H., Oshima, Y. 2017 Diuron causes sinking retardation and physiochemical alteration in marine diatoms Thalassiosira pseudonana and Skeletonema marinoi-dohrnii complex. Chemosphere, 175, 200-209. Keywords: Sinking rate; Thalassiosira pseudonana; Skeletonema marinoi-dohrnii complex; Diuron Strain(s): 324  PubMed: 28222374 DOI: 10.1016/j.chemosphere.2017.02.054 Kohira, M., Matsumoto, T., Yamada, Y., Nanba, N., Kado, R. 2017 Effects of hydrogen peroxide on phytoplanktons, seaweed gametophytes, marine harpacticoid copepod and striped barnacle larvae. Sessile Organisms, 34, 1-12 (in Japanese with English summary). Keywords: hydrogen peroxide; marine ecotoxicity; seaweed gametophytes; phytoplankton; zooplankton Strain(s): 235,  324,  586,  1375,  1429  DOI: 10.4282/sosj.34.1 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1,  8,  9,  14,  15,  17,  71,  115,  223,  225,  233,  234,  265,  274,  275,  276,  277,  278,  279,  280,  281,  282,  284,  293,  323,  324,  330,  333,  344,  345,  346,  347,  348,  350,  353,  372,  377,  388,  391,  395,  407,  408,  409,  413,  414,  417,  461,  462,  466,  487,  534,  548,  553,  556,  557,  558,  559,  560,  562,  587,  588,  589,  590,  603,  605,  622,  623,  695,  696,  697,  698,  699,  700,  701,  702,  703,  704,  705,  706,  707,  708,  709,  710,  711,  712,  713,  714,  715,  716,  741,  765,  766,  767,  805,  837,  997,  1001,  1002,  1003,  1004,  1005,  1006,  1007,  1009,  1011,  1016,  1017,  1044,  1045,  1046,  1047,  1302,  1303,  1324,  1327,  1330,  1339,  1340,  1349,  1353,  1370,  1375,  1376,  1379,  1383,  1384,  1385,  1386,  1387,  1391,  1392,  1393,  1395,  1398,  1399,  1400,  1401,  1699,  1700,  1730,  1813,  1815,  1816,  1826,  1827,  1831,  1862,  1863,  1864,  1865,  1874,  1963,  1964,  1965,  1974,  1975,  1976,  2142,  2143,  2144,  2145,  2147,  2148,  2300,  2331,  2332,  2351,  2363,  2364,  2365,  2369,  2370,  2376,  2506,  2533,  2534,  2535,  2536,  2537,  2590,  2633,  2668,  2689,  2690,  2691,  2693,  2694,  2696,  2697,  2707,  2716,  2717,  2718,  2720,  2722,  2723,  2725,  2726,  2729,  2730,  2731,  2732,  2770,  2771,  2772,  2773,  2839,  2840,  2841,  2842,  2843,  2844,  2859,  2872,  2878,  2890,  2899,  3391,  3689,  3690,  3691  Fuchida, S.,Yokoyama, A., Fukuchi, R., Ishibashi, J., Kawagucci, S., Kawachi, M., Koshikawa, H. 2017 Leaching of metals and metalloids from hydrothermal ore particulates and their effects on marine phytoplankton. ACS Omega, 2, 3175–3182. Keywords: Geological materials; Microbiology; Separation science; Toxicology; Water pollution Strain(s): 324  PubMed: 30023687 DOI: 10.1021/acsomega.7b00081 Shimasaki, Y., Tsuyama, M., Tasmin, R., Khanam, M. R. M., Qiu, X., Oshima, Y. 2015 Comparative study of toxicological responses of chlorophyllrelated parameters in the marine diatom Skeletonema sp. exposed to tributyltin and Irgarol. Jpn. J. Environ. Toxicol., 18, 1-10. Keywords: tributyltin; Irgarol; diatom; chlorophyll; photosynthesis Strain(s): 324  DOI: 10.11403/jset.18.1 Naito, K., Sakai, S., Fujinaga, S., Yoshinaga, I., Tanaka, M. 2014 Evolution of water quality at a tidal flat in the Ariake Sea by Algal Growth Potential test. J. life. Environ. Sci., 6, 17-28 (in Japanese with English summary). Keywords: Ariake Sea; AGP test; Nutrient; Iron; Microalgae Strain(s): 1,  324  Qiu, X., Shimasaki, Y., Yoshida, Y., Matsubara, T., Yamasaki, Y., Kawaguchi, M., Honda, M., Mouri, K., Nakajima, Y., Tasmin, R., Kuno, K., Kawamura, Y., Honjo, T., Oshima, Y. 2014 Allelopathic effects of Skeletonema spp. may influence interspecific competition and bloom formation of co–occurring harmful flagellates. J. Fac. Agr., Kyushu Univ., 59, 373-382. Strain(s): 1,  10,  324  Enomoto, J., Kimura, T., Nomura, Y., Kabayama, J., Maita, M. 2013 The safety of febantel for tiger puffer and its environmental impact. Nippon Suisan Gakkaishi, 79, 400-409 (in Japanese with English summary). Keywords: tiger puffer; febantel; Heterobothrium okamotoi Strain(s): 324  DOI: 10.2331/suisan.79.400 Onduka, T., Ojima, D., Ito, M., Ito, K., Mochida, K., Fujii, K. 2013 Toxicity of degradation products of the antifouling biocide pyridine triphenylborane to marine organisms. Arch. Environ. Contam. Toxicol., 65, 724-732. Strain(s): 324  PubMed: 23929384 DOI: 10.1007/s00244-013-9945-x Onduka,T., Ojima, D., Ito, M., Ito, K., Mochida, K., Fujii, K. 2013 Toxicity of the antifouling biocide Sea-Nine 211 to marine algae, crustacea, and a polychaete. Fish. Sci., 79, 999-1006. Keywords: Risk assessment; Ecological toxicity; Coastal area of Japan; Perinereis nuntia; Sediment toxicity test; No-observed-effect concentration Strain(s): 324  DOI: 10.1007/s12562-013-0678-6 Qiu, XC., Yamasaki, Y., Shimasaki, Y., Gunjikake, H., Honda, M., Kawagushi, M., Matsubara, T., Nagasoe, S., Etoh, T., Matsui, S., Honjo, T., Oshima, Y. 2012 Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact. Mar. Ecol. Prog. Ser., 446, 107-118. Keywords: Heterosigma akashiwo; Akashiwo sanguinea; Allelopathy; Polysaccharide−protein complex; Cell contact; Growth interaction; Growth simulation Strain(s): 10,  324  DOI: 10.3354/meps09476 Mochida, K., Onduka, T., Amano, H., Ito, M., Tanaka, H., Fujii, K. 2012 Use of species sensitivity distributions to predict no-effect concentrations of an antifouling biocide, pyridine triphenylborane, for marine organisms. Mar. Pollut. Bull., 64, 2807-2814. Keywords: Algal growth inhibition test; Bayesian inference; Crustacean immobilization test; Fish acute toxicity; Primary risk analysis Strain(s): 324  PubMed: 23044030 DOI: 10.1016/j.marpolbul.2012.09.007 Onduka, T., Kakuno, A., Kono, K., Ito, K., Mochida, K., Fujii, K. 2012 Toxicity of chlorothalonil to marine organisms. Fish. Sci., 78, 1301-1308. Keywords: Antifouling biocides; Toxicity; Early-life-stage Toxicity test; Predicted no-effect concentration Strain(s): 324  DOI: 10.1007/s12562-012-0562-9 Onduka, T., Ojima, D., Kakuno, A., Mochida, K., Ito, K., Koyama, J., Fujii, K. 2012 Nitrated polycyclic aromatic hydrocarbons in the marine environment: acute toxicities for organisms at three trophic levels. Jpn. J. Environ. Toxicol., 15, 1-10. Keywords: alga; crustacean, fish, nitro-PAHs; predicted no effect concentrations Strain(s): 324  DOI: 10.11403/jset.15.1 Yamasaki, Y., Ohmichi, Y., Hirose, M., Shikata, T., Shimasaki, Y., Oshima, Y., Honjo, T. 2012 Low molecular weight allelochemicals produced by the diatom, Skeletonema costatum. Thalassas, 28, 9-17. Keywords: Allelochemicals; Diatom; Growth inhibition; Heterosigma akashiwo; Raphidophyt; Skeletonema costatum Strain(s): 10,  324  Onduka, T., Mochida, K., Harino, H., Ito, K., Kakuno, A., Fujii, K. 2010 Toxicity of metal pyrithione photodegradation products to marine organisms with indirect evidence for their presence in seawater. Arch. Environ. Contam. Toxicol., 58, 991-997. Strain(s): 324  PubMed: 19967345 DOI: 10.1007/s00244-009-9430-8 Yamasaki, Y., Nagasoe, S., Tameishi, M., Shikata, T., Zou, Y., Jiang, Z., Matsubara, T., Shimasaki, Y., Yamaguchi, K., Oshima, Y., Honjo, T. 2010 The role of interactions between Prorocentrum minimum and Heterosigma akashiwo in bloom formation. Hydrobiologia, 641, 33-44. Keywords: Allelopathy; growth and interaction; Heterosigma akashiwo; Prorocentrum minimum; Reactive oxygen species; Skeletonema costatum Strain(s): 1,  10,  324  DOI: 10.1007/s10750-009-0052-y Yamasaki, Y., Ohmichi, Y., Shikata, T., Hirose, M., Shimasaki, Y., Oshima, Y., Honjo, T. 2010 Species-specific allelopathic effects of the diatom Skeletonema costatum. Thalassas, 27, 21-32. Keywords: Allelopathy; growth inhibition; growth promotion; Heterosigma akashiwo; low molecular weight allelochemicals; Skeletonema costatum; species-specific effect Strain(s): 10,  324  Yamasaki, Y., Shikata, T., Nukata, A., Ichiki, S., Nagasoe, S., Matsubara, T., Shimasaki, Y., Nakao, M., Yamaguchi, K., Oshima, Y., Oda, T., Ito, M., Jenkinson, I. R., Asakawa, M., Honjo, T. 2009 Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community. ISME J., 3, 808-817. Keywords: allelopathy; growth inhibition; growth stimulation; harmful algal blooms (HABs); Heterosigma akashiwo; Skeletonema costatum Strain(s): 10,  324  PubMed: 19322242 DOI: 10.1038/ismej.2009.24 Kooistra, W. H. C. F., Sarno, D., Balzano, S., Gu, H., Andersen, R. A., Zingone, A. 2008 Global diversity and biogeography of Skeletonema species (Bacillariophyta). Protist, 159, 177-193. Keywords: biogeography; cryptic species; distribution; LSU rDNA; phytoplankton; Skeletonema costatum Strain(s): 16,  17,  223,  323,  324  PubMed: 18042429 DOI: 10.1016/j.protis.2007.09.004 Oh, S. J., Kim, D.-I., Sajima, T., Shimasaki, Y., Matsuyama, Y., Oshima, Y., Honjo, T., Yang, H.-S. 2008 Effects of irradiance of various wavelengths from light-emitting diodes on the growth of the harmful dinoflagellate Heterocapsa circularisquama and the diatom Skeletonema costatum. Fish. Sci., 74, 137-145. Keywords: Heterocapsa circularisquama; irradiance; light wavelengths; light-emitting diode; Skeletonema costatum Strain(s): 324  DOI: 10.1111/j.1444-2906.2007.01503.x Matsubara, T., Nagasoe, S., Yamasaki, Y., Shikata, T., Shimasaki, Y., Oshima, Y., Honjo, T. 2008 Inhibitory effects of centric diatoms on the growth of the dinoflagellate Akashiwo sanguinea. Nippon Suisan Gakkaishi, 74, 598-606 (in Japanese with English summary). Keywords: Akashiwo sanguinea; allelopathy; growth inhibition; centric diatoms Strain(s): 324  DOI: 10.2331/suisan.74.598 Fukao, T., Nishijima, T., Yamaguchi, H., Adachi, M. 2007 Utilization of urea by the 6 species of red tide phytoplankton. Bull. Plankton Soc. Japan, 54, 1-8 (in Japanese with English summary). Keywords: growth; red tide; phytoplankton; urea; uptake kinetics Strain(s): 1,  6,  237,  323,  324,  605  Yamasaki, Y., Nagasoe, S., Matsubara, T., Shikata, T., Shimasaki, Y., Oshima, Y., Honjo, T. 2007 Allelopathic interactions between the bacillariophyte Skeletonema costatum and the raphidophyte Heterosigma akashiwo. Mar. Ecol. Prog. Ser., 339, 83-92. Keywords: Allelopathy; Bacillariophyceae; Skeletonema costatum; Raphidophyceae; Heterosigma akashiwo; Bi-algal culture; Growth inhibition Strain(s): 10,  324  DOI: 10.3354/meps339083 Nagasoe, S., Toda, S., Shimasaki, Y., Osima, Y., Uchida, T., Honjo, T. 2006 Growth inhibition of Gyrodinium instriatum (Dinophyceae) by Skeletonema costatum (Bacillariophyceae). Afr. J. Mar. Sci., 28, 325-329. Keywords: allelopathy; Gyrodinium instriatum; Skeletonema costatum; species interaction Strain(s): 324  DOI: 10.2989/18142320609504171 Hirano, T., Masho, R., Koizumi, D., Tabata, S., Suzuki, K., Sakabe, M., Nakano, Y., Sato, N., Sudo, R. 2006 Ecotoxicity testing of bioremediation agents for shoreline oil-spill clean up. Jpn. J. Environ. Toxicol., 9, 115-131. Keywords: ecotoxicity; bioremediation; oil-spill; acute toxicity; marine organisms Strain(s): 324  DOI: 10.11403/jset.9.115 Yamaguchi, H., Nishijima, T., Nishitani, H., Fukami, K., Adachi, M. 2004 Organic phosphorus utilization and alkaline phosphatase production of 3 red tide photoplankton. Nippon Suisan Gakkaishi, 70, 123-130 (in Japanese with English summary). Keywords: Karenia mikimotoi; Skeletonema costatum; Heterosigma akashiwo; red tide phytoplankton; organic phosphorus; alkaline phosphatase Strain(s): 5,  324  DOI: 10.2331/suisan.70.123 Okumura, C., Hasegawa, H., Mizumoto, H., Maki, T., Ueda, K. 2004 Size fractionation of iron compounds in phytoplankton cultures in the presence of chelating ligands. Bunseki Kagaku, 53, 1215-1221 (in Japanese with English summary). Keywords: iron; speciation; chelating ligands; phytoplankton growth Strain(s): 324  DOI: 10.2116/bunsekikagaku.53.1215 Okumura, Y., Koyama, J., Takaku, H., Satoh, H. 2001 Influence of organic solvents on the growth of marine microalgae. Arch. Environ. Contam. Toxicol., 41, 123-128. Strain(s): 6,  324  PubMed: 11462135 DOI: 10.1007/s002440010229 Mitsutani, A., Yamasaki, I., Kitaguchi, H., Kato, J., Ueno, S., Ishida, Y. 2001 Analysis of algicidal proteins of a diatom-lytic marine bacterium Pseudoalteromonas sp. strain A25 by two-dimensional electrophoresis. Phycologia, 40, 286-291. Strain(s): 324  DOI: 10.2216/i0031-8884-40-3-286.1 Lee, S, Kato, J, Takiguchi, N. Kuroda, A., Ikeda, T., Mitsutani, A., Ohtake, H. 2000 Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28. Appl. Environ. Microbiol., 66, 4334-4339. Strain(s): 324  PubMed: 11010878 DOI: 10.1128/AEM.66.10.4334-4339.2000 Fukami, K., Matsumoto, J., Kadota, T., Nakano, Y. 2000 Seasonal fluctuations of water quality of deep sea water and the relationship to the growth potential of a microalga. Deep Ocean Water Research, 1, 27-31 (in Japanese with English summary). Keywords: Deep seawater; water quality; inorganic nutrients; AGP; seasonal fluctuation; upwelling Strain(s): 324  DOI: 10.11174/dowas2000.1.1_27 Okamura, K. 1992 Growth characteristics of Skeletonema costatum for algal growth potential. Kanagawa-ken Kankyô Kagaku Sentâ Kenkyû Hôkoku, 15, 12-15 (in Japanese with English title). Strain(s): 324  Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited. PAGE TOP 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, JAPAN TEL +81-29-850-2556 FAX +81-29-850-2587 Send us an e-mail () FAQ Privacy policy ©National Institute for Environmental Studies. All Rights Reserved. Since 1 October 2001