ビートダウンセンター北

× {{flash.message}} Toggle navigation Toggle navigation 日本語 | English 新規登録ログイン 細川 まゆ子 ホソカワ マユコ  (Mayuko Hosokawa) 更新日: 01/31 ホーム研究キーワード研究分野学歴論文MISC書籍等出版物講演・口頭発表等担当経験のある科目(授業)所属学協会Works(作品等)共同研究・競争的資金等の研究課題 論文 査読有り 2007年6月 Magnetometric evaluation of the effects of man-made mineral fibers on the function of macrophages using the macrophage cell line RAW 264.7 INDUSTRIAL HEALTH Kaori Shibata, Yuichiro Kudo, Masashi Tsunoda, Mayuko Hosokawa, Yasuhiro Sakai, Makoto Kotani, Yoshiharu Aizawa 巻 45 号 3 開始ページ 426 終了ページ 436 記述言語 英語 掲載種別 研究論文（学術雑誌） DOI 10.2486/indhealth.45.426 出版者・発行元 NATL INST OCCUPATIONAL SAFETY & HEALTH, JAPAN The toxic effects of man-made mineral fibers (MMMFs) have been evaluated by cell magnetometry using alveolar macrophages (AMs). Recently, on the other hand, the murine macrophage cell line, RAW 264.7, became available and has been used as an in vitro model of AMs. The objective of this study was to determine whether or not cell magnetometry using RAW 264.7 cells can be used to evaluate the toxic effects of MMMFs. RAW 264.7 cells were exposed to one of the MMMFs, potassium octatitanate (PT) or silicon carbide whisker (SiC) at 0, 20, 40 and 60 mu g/ml, or chrysotile as a positive control at 0, 15,20 and 25 mu g/ml. The toxic effects of fibers were evaluated by cell magnetometry and LDH assay. For this comparison, AMs were also exposed to chrysotile fibers (CF). In the RAW 264.7 cells exposed to PT 20, 40, 60 or SiC 20, 40, 60, CF 15, 20 and 25 mu g/ml, significant delayed relaxation were observed compared with the respective control. In the LDH assay, significant increases in LDH in the supernatant of the cells exposed to PT 20, 40, 60, SiC 20, 40, 60 and CF 15, 20, 25 mu g/ml were observed. In AMs exposed to CF 20, 25 mu g/ml significant delayed relaxation and significant increases in LDH compared with the control were observed. The levels of MMMFs that induced significant differences were similar for cell magnetometry and LDH. The levels of CF that induced significant differences in cell magnetometry and LDH were identical for RAW 264.7 cells and AMs. Our results suggest that cell magnetometry using RAW 264.7 cells is adequate to evaluate the cytotoxicity of exposure to MMMFs. リンク情報 DOIhttps://doi.org/10.2486/indhealth.45.426PubMedhttps://www.ncbi.nlm.nih.gov/pubmed/17634692Web of Sciencehttps://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000247738700008&DestApp=WOS_CPL ID情報 DOI : 10.2486/indhealth.45.426ISSN : 0019-8366PubMed ID : 17634692Web of Science ID : WOS:000247738700008 エクスポート BibTeX RIS 論文リストへ メニュー マイポータル 研究ブログ資料公開 共著者の一覧 まだ共著者が1人も登録されていません。 {{coauthor.Related.name_str}} {{coauthor.Related.last_modified}} 更新 limit"> もっとみる 2017 researchmap 利用規約